Biotinylated- hyaluronan binding protein
WebApr 29, 2024 · Biotinylated hyaluronan binding protein (b-HABP) antibody (amsbio, AMS.HKD-BC41, 1:200 in antibody diluent) was added to the slides and incubated overnight at 4 °C. WebAug 19, 2013 · Abstract. Hyaluronan (HA) is widely detected in biological samples and its concentration is most commonly determined by the use of a labeled specific HA binding …
Biotinylated- hyaluronan binding protein
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WebJul 30, 2024 · Hyaluronan Binding Protein Assay. To localize hyaluronan (HA) in histologic sections, we performed a fluorescent HA binding protein assay ... After a rinse in PBS, biotinylated-HABP (1:100 in normal goat serum, Millipore-Sigma) was added to the slides for 1 h at room temperature. Next, the slides were washed with PBS 4 times and … WebApr 12, 2024 · Hyaluronan binding proteins are of two types; those with link modules, and those without. Link modules are 100 amino acid (aa) HA and proteinbinding sequences that contain two αhelices and two antiparallel βsheets (1, 3). There are three categories of link modulecontaining proteins.
WebAug 19, 2013 · Abstract. Hyaluronan (HA) is widely detected in biological samples and its concentration is most commonly determined by the use of a labeled specific HA binding protein (aggrecan G1-IGD-G2, HABP), employing membrane blotting and sandwich enzyme-linked immunosorbent assay (ELISA)-like methods. WebJan 31, 2011 · Knudson, CB; Knudson, W. Hyaluronan-binding proteins in development, tissue homeostasis, and disease. FASEB J 1993, 7 (13), 1233–1241. [Google Scholar] Weigel, PH; Hascall, VC; Tammi, M. Hyaluronan synthases. ... HA was detected using biotinylated HABP (d, e, f) as described previously . CD44 immunohistochemistry (g, h, …
WebJan 1, 2002 · Growing evidence for intracellular hyaluronan in tissues in vivo, together with evidence of intracellular hyaluronan-binding molecules, prompted us to examine hyaluronan distribution and uptake, and hyaluronan-binding sites within cells and their relationship to cell proliferation in vitro using a biotinylated hyaluronan-binding protein … WebBiotinylated hyaluronan-binding protein was from Merck. Purified NA/LE mouse anti-CD44 (clone 515) was obtained from BD Biosciences Pharmingen. Anti-CD44 antibody (156-3C11), ERK inhibitor (U0126), and anti-phospho-ERK1/2 mAb were obtained from Cell Signal. nHA was obtained from Sigma. Hyaluronan oligosaccharides (oligomers) were …
WebHyaluronan binding protein (HABP) is a ubiquitously present glycoprotein having specific affinity towards hyaluronic acid (HA). HA, also known as Hyaluronan, is a naturally …
Webadjective. bio· tin· y· lat· ed ˌbī-ə-ˈti-nə-ˌlā-təd. : combined with biotin. The PCR reaction uses biotinylated primers to define a sequence of approximately 207 nucleotides within … solicitors in sawston cambridgeWebFeb 1, 2000 · The biotinylated HABP–link protein complex is applied to tissue sections, and binding of the complex to tissue hyaluronan is visualized by enzymatic precipitation of chromogenic substrates. View ... solicitors in sale cheshireWebHyaluronan-binding protein in ductal branching morphogenesis, and absence is associated endothelial cell morphogenesis. J. Cell Biol. 119, 643-652. with substantial alterations in the branching. ... Matrix 12, 397-403. Identification of hyaluronan binding proteins using a biotinylated hyaluronan Sugimura, Y., Cunha, G. and Bigsby, R. … smakus southportWebFigure 7 A representative overlaid sensorgram for HA-binding kinetics of bovine link protein. Biotinylated hyaluronan (102 RU) was captured by streptavidin on the sensor chip. Various concentrations of link protein … solicitors in shaftesbury dorsetWebFigure Legend Snippet: Hyaluronan-rich septa are largely unchanged throughout mammary gland development. Immunofluorescence microscopy for hyaluronic acid binding protein (HABP; green) and DAPI nuclear stain identifying hyaluronan (HA) septa within the mammary glands derived from nulliparous (6-week, proestrus, metestrus), pregnant (P … smak-tak polish restaurant chicagoWebBiotinylated Hyaluronan Binding Protein, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - … solicitors in selly oakWebHyaluronic Acid Binding Protein, Bovine Nasal Cartilage, Biotinylated; find Sigma-Aldrich-385911 MSDS, related peer-reviewed papers, technical documents, similar … solicitors in saxmundham suffolk