Chimeric chalcone synthase
WebChalcone Synthase Gene. Chalcone synthase (CHS) is the first committed enzyme in flavonoid biosynthesis (Fig. 1) and catalyzes the stepwise condensation of three acetate … WebJSTOR Home
Chimeric chalcone synthase
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WebMar 21, 1997 · Transgenic Petunia plants with a chsA coding sequence under the control of a 35S promoter sometimes lose endogene and transgene chalcone synthase activity and purple flower pigment through posttranscriptional chsA RNA degradation. In these plants, shorter poly(A)+ and poly(A)− chsA RNAs are found, and a 3′ end–specific RNA … WebA chimeric gene was constructed containing the light‐inducible chalcone synthase (chs) promoter from Antirrhinum majus, the neomycin phosphotransferase structural sequence from Tn5 as a reporter gene (NPTII) and the termination region from chs gene 1 from Petroselinum hortense.
WebSep 1, 2016 · DPPH (1,1–diphenyl-2-picrylhydrazyl) radical scavenging activity was measured for all the chalcones (2a–2e).Among them, compounds 2a, 2b, 2c, 2d and 2e … WebIntroduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans. C. Napoli , C. Lemieux , and R. Jorgensen The Plant Cell. 1990. Find this article
WebThe primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin. Catalytic activity 4-coumaroyl-CoA + 2 H+ + 3 malonyl-CoA = 2',4,4',6'-tetrahydroxychalcone + 3 CO2 + 4 CoA PROSITE-ProRule annotation Chalcone synthase or naringenin-chalcone synthase (CHS) is an enzyme ubiquitous to higher plants and belongs to a family of polyketide synthase enzymes (PKS) known as type III PKS. Type III PKSs are associated with the production of chalcones, a class of organic compounds found mainly in plants as natural … See more CHS catalysis serves as the initial step for flavonoid biosynthesis. Flavonoids are important plant secondary metabolites that serve various functions in higher plants. These include pigmentation, UV protection, fertility, … See more Subunits CHS exists as a homodimeric protein with each monomer approximately 42-45 kDa in size. Each … See more Metabolic CHS is noncompetitively inhibited by flavanoid pathway products such as naringenin and chalcone naringenin. Despite lack of direct evidence in vivo, flavonoids are believed to accumulate in the cytosol to a … See more CHS belongs to a broader class of enzymes known as type III PKSs. Being the first enzyme of its type to be discovered, all other members are often labeled as “CHS-like.” Most or all of the divergent CHS-like enzymes characterized have arisen from … See more 4-coumaroyl-CoA and three units of malonyl-CoA are converted into three molecules of carbon dioxide, four molecules of coenzyme A and one unit of naringenin chalcone. See more The first step involves a transfer of a coumaroyl moiety from a 4-coumaroyl-CoA starter molecule to Cys164. Next, a series of condensation reactions of three acetate units from malonyl-CoA occurs, each proceeding through an acetyl-CoA carbanion derived … See more CHS, as the first committed step in the flavonoid pathway, facilitate production of flavanoids, isoflavonoid-type phytoalexins and other metabolites to protect the plant from stress. CHS … See more
WebJun 1, 1992 · We developed an Agrobacterium-mediated leaf disk transformation method for chrysanthemum. We introduced a chimeric chalcone synthase (CHS) gene isolated from chrysanthemum into cv. Moneymaker (pink type) to produce white-flowered plants.
WebIn Basic medium (base catalysis), the chalcone is produced from the aldol product via dehydration in (an enolate) - mechanism, while in acidic medium (acid catalysis, it is produced via (an... react export syntaxWebJan 1, 2006 · Chalcone synthase (CHS; EC 2.3.1.74) is a key enzyme in anthocyanin biosynthesis. ... tobacco plants were transformed with a chimeric construct containing expression cassettes for neomycin ... react exportsWebDec 13, 2024 · The chalcone synthase superfamily of type III polyketide synthases. ... A study utilizing both mutant and BBS/STS chimeric enzymes attempted to elucidate the structural basis for this unusual substrate … react export json to csvWebFeb 15, 2024 · Chalcone synthase (CHS) is the key enzyme in the flavonoids biosynthesis pathway, and has been proved to monitor the changes to drought stress tolerance. In this work, we overexpressed a CHS gene ... react export web componentWebApr 1, 1990 · Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans. C. Napoli, C. Lemieux, R. … how to start esports teamWebA chimeric gene was constructed containing the light-inducible chalcone synthase (chs) promoter from Antirrhinum majus, the neomycin phosphotransferase structural sequence … how to start ethereum miningWebOct 21, 2013 · The I locus is a 27-kb inverted repeat cluster of chalcone synthase genes CHS1-3-4 that mediates siRNA down-regulation of CHS7 and CHS8 target mRNAs during seed development leading to yellow seed coats lacking anthocyanin pigments. Here, we report small RNA sequencing of ten stages of seed development from a few days post … how to start essential oil business in india