WebCircRNA pull down and miRNA deep-sequencing demonstrated the binding of miR-92b-3p and circCDYL. Interestingly, circCDYL did not act as miR-92b-3p sponge, but was degraded in miR-92b-3p-dependent silencing manner. Clinically, expression of circCDYL and miR-92b-3p was associated with clinical outcome of HER2+ breast cancer patients. WebThe Endogenous circRNA_0000429 pull-down by AGO2 was enriched in cells transfected with miRNA-1197 . To investigate the function of miRNA-1197 with circRNA_0000429, a circRNA-specific probe was used to pull down its interactive miRNA-1197s. circRNA_0000429 precipitation complex performed the enrichment of miRNA-1197 ( …
云序教您如何应用RIP和RNA pull down技术—研究RNA分子互作机 …
WebRNA pulldown, mass spectrometry, chromatin immunoprecipitation, RNA-binding protein immunoprecipitation, chromatin isolation by RNA purification, electrophoretic mobility shift assay, and luciferase reporter assays were used to explore the molecular sponge role of the circRNA (via miRNA binding), and the interaction between circRNA and … WebOct 22, 2024 · We identified miR-370-5P as a circUBE2J2-related microRNA by using biotin-labeled circUBE2J2 probe to perform RNA antisense purification (RAP) assay in … linstowsgate 3
CircRTN4 promotes pancreatic cancer progression through a novel …
WebOct 5, 2024 · CircRNA are known as micro RNA (miRNA) sponges due to their ability to bind to miRNA molecules and influence their function. They play a key role in the … WebThe RNA pulldown assay selectively extracts protein-RNA complexes from the sample. In general, RNA pulldown assays take advantage of high-affinity tags (such as biotin, etc.) The RNA probe can be biotinylated to complex with proteins in the cell lysate and then purified using agarose or magnetic beads. WebAug 14, 2024 · circRNA在乳腺癌发展过程中有巨大的调控潜能,在乳腺癌(BC)细胞增殖、迁移、侵袭等方面发挥重要作用。 然而,在BC中,circRNAs与自噬的关系以及自噬相关的circRNAs在乳腺癌临床诊断和治疗中的作用仍然需要探索。 展开全文 声明:该文观点仅代表作者本人,搜狐号系信息发布平台,搜狐仅提供信息存储空间服务。 首赞 阅读 () 我来 … linstroff 57