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D3922 thermo fisher scientific

WebJan 22, 2024 · The level of LDs was assayed using BODIPY 493/503 (#D3922, Thermo Fisher Scientific) according to the manufacturer's protocol. 2.5. Western blot. Western blot was performed as previously described [24]. In brief, proteins in the cell lysate or supernatants were resolved on 4%–12% Criterion XT Bis-Tris gels (#3450124, Bio-Rad, … WebJul 26, 2024 · BODIPY lipid staining of 3D-cultured HOF spheroids was performed as described previously. 29, 31, 32 Briefly, 4% paraformaldehyde-fixed 3D spheroids were incubated in a mixture of 0.1% BODIPY (#D3922; Thermo Fisher Scientific), 0.1% DAPI (#D523; Doujin), and 0.1% phalloidin (#20553; Funakoshi) in PBS containing 3% bovine …

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WebNov 8, 2024 · The cells were co-stained with BODIPY™ 493/503 for lipid droplets (D3922; Thermo Fisher Scientific), LysoTracker™ Deep Red (L12492; Thermo Fisher Scientific), or MitoTracker™ (M7510; Thermo Fisher Scientific). Nuclei were blue-stained with 4′,6-diamidino-2-phenylindole (DAPI). Tumor xenograft. All animal study procedures were … WebMay 14, 2024 · Most recently, to understand the molecular etiology of thyroid-associated orbitopathy, our group utilized the 3D cell culture of human orbital fibroblasts, and found that hypoxia-inducible factor... black and gold merry christmas https://mjmcommunications.ca

Lipophagy mediated glucose-induced changes of lipid

WebFeb 8, 2013 · This Thermo Scientific full-size Ultrapure mixed bed ion exchange hose nipple cartridge is an economical way to purify water volumes up to 75L/hour. It is constructed of 100% virgin polypropylene cartridge with superior quality resin. The color change indicator identifies when resin is exhausted. It is designed for non-pressurized … Web493/503 (D3922; Thermo Fisher Scientific) for 30min, followed by thrice PBS washes. The IEC were observed with a laser scan- ... (Thermo Fisher Scientific) and blocked with 8% (w/v) skimmed milk in Tris buffered saline þ Tween 20 (TBST) buffer (20m M Tris–HCl, pH7·5,150mM sodiumchloride,0·1%Tween20)atroomtemper- WebSep 1, 2024 · Briefly, HepG2 cells were fixed with 4% paraformaldehyde for 10 min, then washed three times with phosphate buffer saline (PBS). After washing, cells were incubated with BODIPY 493/503 (D3922, Thermo Fisher Scientific, MA, USA) at room temperature for 1 h, followed by three washes with PBS. black and gold mesh dress

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D3922 thermo fisher scientific

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WebMeasurement Range: -50 to +392°F/-45 to +200°C NSF® Certified One-button operation 3 mm tip 5"/12.7 cm stem Shatterproof Food-safe ABS plastic with BioCote® Stainless … WebJan 16, 2024 · Briefly, 2D or 3D 3T3-L1 cells were incubated in a mixture of 0.2% BODIPY (# D3922, Thermo Fisher Scientific, Waltham, MA, USA), Alexa Fluor 594 phalloidin (# 20553, Funakoshi, Tokyo, Japan), and DAPI (# D523, Dojindo, Kumamoto, Japan), at 1:1000 dilutions in PBS for 1 hr, and thereafter fixed in 4% paraformaldehyde in PBS for …

D3922 thermo fisher scientific

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WebCatalog Number D3922 Product Name 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY® 493/503) CAS Number / Name 121207-31-6 / Boron, [2-[1 … WebMay 14, 2024 · To analyze their lipid droplet formation for the 3D culture, the organoids were transferred to 6 super-low attachment well dishes, incubated in 0.2% BODIPY (# D3922, Thermo Fisher Scientific) in PBS for 1 hr, and then fixed in 4% paraformaldehyde (PFA) in PBS for 10 min at room temperature.

WebApr 20, 2024 · To assess the viability of the adipocytes on-chip, a live/dead-assay based on fluorescein diacetate (FDA; F1303, Thermo Fisher Scientific) and propidium iodide (PI; P3566, Thermo Fisher Scientific ... WebAug 1, 2024 · For BODIPY lipid staining [6–8], 4% paraformaldehyde fixed 3D sphenoids were incubated in 0.1% BODIPY (# D3922, Thermo Fisher Scientific), 0.1% DAPI (#D523, Doujin, Japan) and 0.1% phalloidin (#20,553, Funakoshi, Japan) in phosphate buffered saline containing 3% bovine serum albumin for 3 hrs.

WebFeb 24, 2024 · Finally, BODIPY493/503 (#D3922, Thermo Fisher Scientific, USA) staining was performed to check the lipid droplet formation. ... USA) according to the manufacturer’s instruction. SYBR Green qPCR Master Mix (#K0251, Thermo Fisher Scientific, USA) was used for quantitative real-time PCR amplification with a CFX96 real-time PCR detection … WebJan 9, 2024 · The BODIPY 493/503 (D3922; Thermo Fisher Scientific, USA) staining was performed for primary IECs, as described by Wei et al. . The laser scanning confocal microscope (Leica Microsystems, Germany) …

WebApr 14, 2024 · C1-Bodipy C12 500/510 (Thermo Fisher, D3823) ... (ThermoFisher, D3922) for 20 min at 37 °C with agitation. Samples were then washed twice with PBS and resuspended in PBS and then kept on ice ...

WebJul 23, 2024 · Thermo Fisher: Cat #D3922: Donkey anti-mouse IgG (H + L) secondary antibody alexa Fluor® 488 conjugate: Thermo Fisher: ... (D3922, Thermo Fisher Scientific, California, USA) staining solution in the dark for 15 min at 37°C. After washing 3 times with PBS, cells were stained with theirs surface markers for 30min at 4°C. Data … black and gold metal lamp shadeWebSU24 - Scientific research and development Uses advised againstNot for consumer use. Details of the supplier of the safety data sheet Manufacturer/Supplier 24 hour Emergency … dave chang microwave cookwareWebDec 12, 2024 · Lipophilic green-fluorescent BODIPY 493/503 (D3922, Thermo Fisher Scientific, Waltham, MA, USA) was used for lipid staining. SkMs were seeded at a density of 1000 cells/cm 2 and treated as indicated. dave chandler interviewWebFeb 9, 2024 · To visualize the accumulation of lipids in response to OA treatment, we used boron-dipyrromethene (BODIPY) 493/503 (D3922, Thermo Fisher Scientific, MA, USA), which labels specifically ... dave champney deathWebThermo Fisher Scientific is dedicated to improving the human condition through systems, consumables, and services for researchers. dave chang colored cookwareWebFeb 1, 2024 · We performed ORO and Bodipy 493/503 (D3922; Thermo Fisher Scientific, Waltham, MA, USA) staining based on the protocols described in our previous research [4, 13]. The ten fields were selected from each sample to qualify relative areas of LDs in ORO by Image J. We used the laser scanning confocal microscope (Leica Microsystems, … black and gold metal leaf wall artWebNov 18, 2024 · DFCP1 is an ER-associated protein with two PI (3)P-binding FYVE domains. In WT cells, myc-DFCP1 formed very small punctate structures ( Figure 1E) ( Axe et al., 2008; Li et al., 2024 ). In SKO cells, the myc-DFCP1 structures appeared highly irregular and significantly enlarged ( Figure 1, E and F). dave champion keto