WebJan 22, 2024 · The level of LDs was assayed using BODIPY 493/503 (#D3922, Thermo Fisher Scientific) according to the manufacturer's protocol. 2.5. Western blot. Western blot was performed as previously described [24]. In brief, proteins in the cell lysate or supernatants were resolved on 4%–12% Criterion XT Bis-Tris gels (#3450124, Bio-Rad, … WebJul 26, 2024 · BODIPY lipid staining of 3D-cultured HOF spheroids was performed as described previously. 29, 31, 32 Briefly, 4% paraformaldehyde-fixed 3D spheroids were incubated in a mixture of 0.1% BODIPY (#D3922; Thermo Fisher Scientific), 0.1% DAPI (#D523; Doujin), and 0.1% phalloidin (#20553; Funakoshi) in PBS containing 3% bovine …
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WebNov 8, 2024 · The cells were co-stained with BODIPY™ 493/503 for lipid droplets (D3922; Thermo Fisher Scientific), LysoTracker™ Deep Red (L12492; Thermo Fisher Scientific), or MitoTracker™ (M7510; Thermo Fisher Scientific). Nuclei were blue-stained with 4′,6-diamidino-2-phenylindole (DAPI). Tumor xenograft. All animal study procedures were … WebMay 14, 2024 · Most recently, to understand the molecular etiology of thyroid-associated orbitopathy, our group utilized the 3D cell culture of human orbital fibroblasts, and found that hypoxia-inducible factor... black and gold merry christmas
Lipophagy mediated glucose-induced changes of lipid
WebFeb 8, 2013 · This Thermo Scientific full-size Ultrapure mixed bed ion exchange hose nipple cartridge is an economical way to purify water volumes up to 75L/hour. It is constructed of 100% virgin polypropylene cartridge with superior quality resin. The color change indicator identifies when resin is exhausted. It is designed for non-pressurized … Web493/503 (D3922; Thermo Fisher Scientific) for 30min, followed by thrice PBS washes. The IEC were observed with a laser scan- ... (Thermo Fisher Scientific) and blocked with 8% (w/v) skimmed milk in Tris buffered saline þ Tween 20 (TBST) buffer (20m M Tris–HCl, pH7·5,150mM sodiumchloride,0·1%Tween20)atroomtemper- WebSep 1, 2024 · Briefly, HepG2 cells were fixed with 4% paraformaldehyde for 10 min, then washed three times with phosphate buffer saline (PBS). After washing, cells were incubated with BODIPY 493/503 (D3922, Thermo Fisher Scientific, MA, USA) at room temperature for 1 h, followed by three washes with PBS. black and gold mesh dress