Webapplied ion exchange resins for the removal of hexavalent chromium from wastewater originating from the leather industry.18 Another study used ion exchange resins for the puri cation of acidic waste streams containing heavy metals such as Cu(II), Zn( ), and Ni( ) in lowand adjusted pH ranges as well as high concentration of NaCl.19 Ion exchange ... WebThis makes High Affinity Ni-Charged Resin the excellent choice for high performance purification of polyhistidine-tagged proteins. High Affinity Ni-Charged Resin is available in 10, 25 and 500 ml lab packs.Note: High temperature heating is not recommended. The agarose melts above 65°C.Total Volume:10 ml settled resin (20 ml 50% slurry)25 ml ...
Ni-NTA Agarose - Qiagen
WebAbstract. Immobilized metal affinity chromatography (IMAC) is a technique primarily used in research and development laboratories to purify proteins containing engineered histidine tags. Although this type of chromatography is commonly used, it can be problematic as differing combinations of resins and metal chelators can result in highly ... WebNi-NTA His•Bind Resin is a high-performance Ni 2+-charged agarose used for rapid one-step purification of proteins containing a His•Tag sequence by metal chelation … greater texas credit union san marcos
Fast, small-scale his-tagged protein purification—TALON Spin …
WebHigh Affinity Ni-Charged Resin FF has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. … WebThis prepacked column is intended for purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). After thorough cell disruption, it is possible to load the unclarified lysate on the column without precentrifugation and filtration of … WebIMAC is a widely-used method for rapidly purifying polyhistidine affinity-tagged proteins, resulting in 100-fold enrichments in a single purification step. The chelators most commonly used as ligands for IMAC are nitrilotriacetic acid (NTA) and iminodiacetic acid (IDA). Once IDA-agarose or NTA-agarose resin is prepared, it can be "loaded" with ... flintstones scream