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Lysm-cre/rosa26-tdtomato

WebWe evaluated two conditional (Csf1r-Cre and LysM-Cre) and two inducible [CX 3 CR1-estrogen receptor-Cre (ERCre) and CD68-rtTA] lines. We assessed Cre activation in six resident lung myeloid populations, as well … WebNov 24, 2024 · Using R26-stop fl/fl-TdTomato and tetOn-GFP reporter lines, we assessed the specificity and efficiency of four commercially available Cre driver lines that are often considered "macrophage specific." We evaluated two conditional (Csf1r-Cre and LysM-Cre) and two inducible [CX 3 CR1-estrogen receptor-Cre (ERCre) and CD68-rtTA] lines. We …

Gcg CreERT2 knockin mice as a tool for genetic manipulation in …

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Smad3 Promotes Cancer‐Associated Fibroblasts Generation via …

WebNov 17, 2024 · D) Lineage tracing strategy of LysM-Cre/ROSA-tdTomato mice, where tdTomato open reading frame (ORF) is linked to a constant CAG promoter, which can … WebJul 6, 2024 · The LysM-Cre is one of the most widely used macrophage Cre driver mice. We therefore, wanted to compare tdTomato expression in tamoxifen treated hCD68-tdTom mice with constitutively activated LyM-Cre-tdTom mice. Cre activation in both hCD68-tdTom and LysM-Cre-tdTom resulted in tdTomato expression in macrophages in the peritoneal … WebStock No: 007909 Protocol 29436: Standard PCR Assay - Gt (ROSA)26Sor (tdTomato-WPRE) Version 2. 2 Notes Melt curve analysis is done using a Roche Light Cycler 480. … hof severin

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Category:骨生物学进展：BRD9介导的染色质重塑通过负反馈机制抑制破骨 …

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Lysm-cre/rosa26-tdtomato

Rosa26 Lsl Tdtomato Mice The Jackson Laboratory Bioz

WebNov 1, 2012 · Insertion of tdTomato, a variant of red fluorescent protein, into the Rosa26 locus of PSCs of various rat strains allows ubiquitous expression of tdTomato. Through … WebNov 1, 2012 · Insertion of tdTomato, a variant of red fluorescent protein, into the Rosa26 locus of PSCs of various rat strains allows ubiquitous expression of tdTomato. Through germline transmission of one Rosa26-tdTomato knock-in embryonic stem cell line, we also obtained tdTomato knock-in rats.

Lysm-cre/rosa26-tdtomato

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Web南模生物. Language: 中文 . English; 日本語; 한국어; 账户设置; 退出 WebDec 1, 2016 · We used LysM-Cre/Rosa26-tdTomato mice in which myeloid cells and their progeny permanently express the Rosa26-Tomato (red fluorescence) reporter. Confocal …

WebWatertown is a town of 24,000 people, halfway between Madison and Milwaukee, with the Rock Rock River coursing through its historic downtown. Watertown is an ideal and … WebFeb 26, 2024 · LysM-Cre mice were provided by Ira Tabas (Columbia University, New York, New York, USA) . Rosa26-loxp-mTomato-stop-loxp-mGFP (Rosa26-mT/mG) reporter mice and Rosa26-loxp-stop-loxp-tdTomato (Rosa26-Ai9) reporter mice were obtained as previously described (refs. 34, 35, respectively). All utilized mice were maintained on a …

WebTamoxifen诱导Cre-ERT2小鼠 使用指南. Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似 … WebMar 3, 2024 · Female lysM-Cre+ / Rpl22 (HA)fl/fl donor mice (8–12 weeks old) were anesthetized (ketamine/xylazine, 100 mg/15 mg/kg i.p) and killed by cervical dislocation. Bone marrow cells from femur and tibia were flushed out with sterile HBSS using a 27.5 gauge needle attached to a 10 ml syringe.

WebSep 17, 2024 · In this study, we crossed Tie2-Cre (Mouse Strain: JAX 004128), Cx3Cr1-Cre (Mouse Strain: 036395-UCD) and LysM-Cre (Mouse Strain: JAX 018956) mice with the Il1r1 r/r line to produce ... 4d LPS induced microglial clustering can be modified by IL-1R1. Utilizing our IL-1R1 reporter mouse, IL-1R1 GR/GR, where tdTomato faithfully tracks all …

WebFeb 5, 2014 · To isolate LysM-lineage AT2 cells for expression profiling, adult (PN70) LysM-Cre > tdTomato mice were euthanized and pulmonary vasculature perfused clear (described above). hof severin gmbhWebThe Jackson Laboratory lysm cre transgenic mice. Lysm Cre Transgenic Mice, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on … hof setsWebMar 16, 2015 · After we crossed Catchup mice with a reporter line expressing high levels of tdTomato from a Cre-activatable CAG promoter in the ROSA26 locus 20 (these mice were termed Catchup IVM-red, as they ... huawei flutter push notification tutorial