Tsingke tse005 2×t5 super pcr mix colony
WebWhen used at the recommended 1X final concentration, the Q5 High-Fidelity Master Mix contains 2 mM Mg ++. Q5 High-Fidelity DNA Polymerase is ... 7,666,645, and … Web4 Use 1 µl of reaction for colony PCR (T5 Taq Polymerase) 5 ... Concentration 2XT5 Super PCR Mix 5ul 1x 10uM Forward Primer 0.4ul 0.4uM 10uM Reverse Primer 0.4ul 0.4uM Template DNA 1ul <1ug
Tsingke tse005 2×t5 super pcr mix colony
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WebPublish time 2024-09-12 17:24. TOROGreen® Yeast colony PCR Kit is used to amplify fragments from the yeast genome as well as transformed plasmids. The same as E coli.colony PCR , PCR amplification directly from a yeast colony without DNA or plasmid extraction from yeast for the robust direct PCR mix. The protocol of this kit is easy,rapid … WebMar 7, 2024 · Pipette 8 µL of LB into PCR tubes and 5 mL (+ antibiotics) into glass culture tubes. Pick a colony off the agar plate and mix it into the LB in a PCR tube. Transfer 5 µL …
Web2×T5 Super PCR Mix（Colony） - tsingke.com.cn http://www.transgenbiotech.com/data/upload/image/20241231/1609386732127122.pdf
Weba 0.2-mL PCR tube containing 10 μLof2×PCRbuffer(New England Biolabs, USA); (2) the PCR tube was boiled for 5 – 20 min ona T100™ Thermal Cycler(Bio-Rad, USA); (3)and ... WebMar 9, 2024 · A 2× T5 Super PCR Mix (Colony) (cat. no. TSE005, Tsingke, Beijing, China) was used to identify the genes of single bacteria. Amplifications were performed in a 25 …
http://tsingke-shop.oss-cn-beijing.aliyuncs.com/TSE008-2%C3%97T5%20Super%20PCR%20Mix%28Basic%29%E8%AF%B4%E6%98%8E%E4%B9%A6V1.1.3.pdf
Webcombined with 100 µL of Binding Buffer). Mix thoroughly. Note. If PCR mixture contains primer-dimers, purification without isopropanol is recommended. However, the yield of … first sports betting site austriaWebCitations & References. PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Simply add template and primers, … campbell county children servicesWeb1.产品不推荐用于菌落和菌落PCR，如有需要推荐使用我公司专用PCR Mix（产品货号：TSE005）。 2.扩增脏模板时，可通过“稀释模板，增加循环数”或“减少模板量，扩大体 … campbell county clerk office hoursWebMCLAB Products. I-5™ 2X Hi-Fi PCR Master Mix. I-5 High-Fidelity DNA Polymerase is an ultra-high fidelity and high processivity enzyme. It produces the most accurate copies of … campbell county clerk office jellico tnWebUSD $119.00. A hot-start 2X PCR master mix with dye. SapphireAmp Fast PCR mix is well-suited for E.coli- based colony PCR, and colony checks can be completed in about 1 hour. … campbell county collision center herreid sdWebmy method: 50 ul h20, pic with a yellow tip a E. coli colony, mix with the water, them heat in the PCR machine at 98ºC, for 10 min, them leave at 4ºc for 5 min. Centrifuge 13000g /10 min, use 2 ... campbell county clerk\u0027s office gillette wyWebSep 8, 2015 · PrimeSTAR Max polymerase (2× Master Mix, Takara Bio) was used to generate both altered plasmids. PCR reactions were digested with DpnI for ~2 hours at 37°C and purified with Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel). 1 μl of the purified product (125–150 ng DNA) was combined with 25 μl NEB 5-alpha chemically competent … campbell county circuit court clerk\u0027s office